Electronic Control of DNA Polymerase Binding and Unbinding to Single DNA Molecules
نویسندگان
چکیده
منابع مشابه
Electronic control of DNA polymerase binding and unbinding to single DNA molecules.
DNA polymerases catalyze template-dependent genome replication. The assembly of a high affinity ternary complex between these enzymes, the double strand-single strand junction of their DNA substrate, and the deoxynucleoside triphosphate (dNTP) complementary to the first template base in the polymerase active site is essential to this process. We present a single molecule method for iterative me...
متن کاملJohn Eid , Molecules Real - Time DNA Sequencing from Single Polymerase
www.sciencemag.org (this information is current as of February 2, 2009 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/323/5910/133 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/1162986/DC1 can be found at:...
متن کاملReal-time DNA sequencing from single polymerase molecules.
Pacific Biosciences has developed a method for real-time sequencing of single DNA molecules (Eid et al., 2009), with intrinsic sequencing rates of several bases per second and read lengths into the kilobase range. Conceptually, this sequencing approach is based on eavesdropping on the activity of DNA polymerase carrying out template-directed DNA polymerization. Performed in a highly parallel op...
متن کاملMolecules Real - Time DNA Sequencing from Single Polymerase
http://www.sciencemag.org/cgi/content/full/323/5910/133 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/1162986/DC1 can be found at: Supporting Online Material found at: can be related to this article A list of selected additional articles on the Science Web sites http://www.sc...
متن کاملBINDING OF THE ANTITUMOR DRUG ADRIAMYCIN TO DNA-HISTONE COMPLEXES
Isotherms of the binding of the anthracycIine antibiotic, adriamycin (adriblastin), to DNA histone complexes was studied by means of spectroscopic analysis. The results indicated that: (a) binding of adriamycin to histones reduced the interaction of histones with DNA, (b) binding of the drug to DNA did not change the binding affinity of histone to DNA and, (c) in the explored binding range...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: ACS Nano
سال: 2009
ISSN: 1936-0851,1936-086X
DOI: 10.1021/nn9000897